Y-SNP-genotyping – a new approach in forensic analysis
R. Lessiga, M. Zoledziewskac, K. Fahrb, J. Edelmanna, M. Kostrzewab, T. Doboszc, W.J. Kleemann
Forensic Science International 154 (2005) 128–136
R. Lessiga, M. Zoledziewskac, K. Fahrb, J. Edelmanna, M. Kostrzewab, T. Doboszc, W.J. Kleemann
Forensic Science International 154 (2005) 128–136
Y-chromosomal DNA polymorphisms, especially Y-STRs are well established in forensic routine case work. The STRs are used for identification in paternity deficiency cases and stain analysis with complicate mixtures of male and female DNA. In contrast, Y-chromosomal SNPs are a new tool in forensic investigations. At present, Y-SNPs are mainly used in molecular anthropology for evolutionary studies. Nevertheless, these markers could also provide very useful information for the analysis of forensic cases. The aim of the presented study was to test Y-SNP-typing for stain analyses using different methods—SNaPshot and MALDI-TOF MS. Both methods are based on the principle of minisequencing. The selected Y-SNP markers are suited to define the most important European haplogroups.
Forensic Sci Int. 2005 Nov 25;154(2-3):128-36. Epub 2004 Nov 23.
PMID: 16182959 [PubMed - indexed for MEDLINE]
Forensic Sci Int. 2005 Nov 25;154(2-3):128-36. Epub 2004 Nov 23.
PMID: 16182959 [PubMed - indexed for MEDLINE]
==========
>> Y-STR은 법과학 사건 조사에서 부계 식별 또는 남성과 여성의 DNA가 섞여있을 경우에 유용하게 이용할 수 있다. STR과는 달리, Y-SNP는 법과학 조사에서 새롭게 이용되는 도구이다. 현재는 주로 인류학의 진화 연구에 이용되고 있다. 그렇지만 이들 마커는 이들은 법과학 사건 분석에도 매우 유용한 정보를 제공해 줄 수 있다. 이 연구의 목표는 다른 방법을 사용하여 흔적에 대한 Y-SNP typing 분석방법 두가지(SNaPshot 과 MALDI-TOF MS)를 테스트해보는 것이다. 두 가지 방법은 minisequencing을 기반으로 하고 있다.
==========
* genotyping of Y-SNPs using the genoSNIP assay based on MALDI-TOF MS is less time consuming.
* MALDI-TOF MS is not as widely distributed, the mass spectrometry has the disadvantage of high starting costs so far.
* Y-SNPs correctly also in samples containing less than 125pg DNA.
* High amount of female DNA did not disturb the PCR reaction of the male part.
* detection of the haplogroups can give additional information in the routine of criminalistics to identify an unknown body or perpetrator.
* A special advantage of the SNPs is the very short length of the fragments, what is very important in particular for the analysis of degraded DNA.
* MALDI-TOF MS is not as widely distributed, the mass spectrometry has the disadvantage of high starting costs so far.
* Y-SNPs correctly also in samples containing less than 125pg DNA.
* High amount of female DNA did not disturb the PCR reaction of the male part.
* detection of the haplogroups can give additional information in the routine of criminalistics to identify an unknown body or perpetrator.
* A special advantage of the SNPs is the very short length of the fragments, what is very important in particular for the analysis of degraded DNA.
'[BT]' 카테고리의 다른 글
| Transcription and Translation 동영상 (0) | 2008.07.01 |
|---|---|
| The Inner Life of The Cell (0) | 2008.07.01 |
| DNA (Deoxyribonucleic acid) (0) | 2008.07.01 |
| DNA sequencing 의 두 가지 방법 (0) | 2008.06.26 |
| PCR song (0) | 2008.01.24 |
